分泌表达pEGF的重组酿酒酵母在断奶仔猪上的应用研究

doi:10.11843/j.issn.0366-6964.2016.05.011

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (5): 944-954.doi: 10.11843/j.issn.0366-6964.2016.05.011

分泌表达pEGF的重组酿酒酵母在断奶仔猪上的应用研究

王蜀金^1，2#，王斌星^1#，郭春华^1* ，柏雪¹，彭忠利¹，张正帆¹，周琳³

(1.西南民族大学生命科学与技术学院，成都 610040； 2.动物遗传育种学国家民委-教育部重点实验室，成都 610040；3.深圳比利美英伟营养饲料有限公司，深圳 518103)

收稿日期:2015-05-23 出版日期:2016-05-23 发布日期:2016-05-23
通讯作者: 郭春华，博士，教授，主要从事分子营养及猪的营养研究，E-mail：531893818@qq.com
作者简介:王蜀金（1988-），男，重庆开县人，硕士生，主要从事分子营养及猪的营养研究，E-mail：137638758@qq.com；王斌星（1990-），男，安徽岳西人，硕士生，主要从事动物营养与饲料科学研究，E-mail：1045302767@qq.com。王蜀金与王斌星为本文共同第一作者
基金资助:
西南民族大学动物科学专业学位建设项目(2014XWD-S0905)

Application Research of Recombinant EGF-expressed Saccharomyces cerevisiae in Early-weaned Piglets

WANG Shu-jin^1，2# ，WANG Bin-xing^1# ，GUO Chun-hua^1* ，BAI Xue¹，PENG Zhong-li¹，ZHANG Zheng-fan¹，ZHOU Lin³

(1.College of Life Science and Technology，Southwest University for Nationalities，Chengdu 610040，China；2.Key Laboratory-Animal Genetics and Breeding of State Ethnic Affairs Commission of Ministry of Education，Chengdu 610040，China；3.Shenzhen Premix Nutrition CO.，LTD，Shenzhen 518103，China)

Received:2015-05-23 Online:2016-05-23 Published:2016-05-23

摘要/Abstract

摘要：

本研究对分泌表达pEGF的重组酿酒酵母在断奶仔猪日粮中的应用效果进行验证。72头21～24日龄的断奶仔猪（(6.10±0.15)kg），随机分为3组（每组4个重复，每个重复6头）：对照组（基础日粮＋228 mL•kg^-1空白培养液）、空载体组（基础日粮＋228 mL•kg^-1空载体）及重组酵母组(基础日粮+228 mL•kg^-1 INVSc1（pYES2-Mfα-spEGF）)。21 d后，每组每栏选取1头仔猪屠宰，分别测定其小肠组织形态、小肠黏膜中IgA、IgG及IgM水平，碱性磷酸酶（ALP）、肌酸激酶（CK）及乳酸脱氢酶（LDH）的酶活力及mRNA转录水平，小肠黏膜中表皮生长因子受体（Epidermal growth factor receptor，EGF-R）的mRNA转录水平。结果表明，与对照组及空载体组相比，重组酵母组INVSc1（pYES2-Mfα-spEGF）能够显著提高断奶仔猪小肠各段（十二指肠、空肠及回肠）黏膜中EGF-R及消化酶（ALP、CK及LDH）的mRNA转录水平（P<0.05），极显著提高CK和LDH的酶活力（P<0.01），显著促进其空肠及回肠黏膜中IgA、IgG及IgM水平（P<0.05），提高其平均日增重（ADG）及降低F/G（P<0.05）。综上，本研究获得的重组酿酒酵母INVSc1（pYES2-Mfα-spEGF）表达的EGF蛋白具有较高的生物学活性，能明显促进断奶仔猪小肠黏膜发育及其免疫功能，具有向养猪生产进一步转化的开发价值。

Abstract:

The application research of recombinant pEGF-expressed S.cerevisiae in early-weaned piglets was verified in the present research.A total of 72 piglets (body weight was (6.10±0.15)kg) weaned at 21-24 day of age were randomly assigned to 3 groups (4 replicates in each group，6 per replicate)：control group (basal diet＋228 mL•kg^-1 culture medium)，empty vector-expressed S.cerevisiae group (basal diet＋228 mL•kg^-1 INVSc1(EV))，and recombinant EGF-expressed S.cerevisiae group (basal diet＋228 mL•kg^-1 INVSc1(pYES2-Mfα-spEGF)).At day 21，one piglet from each pen were randomly chosen to be killed to investigate the morphology，the levels of IgA，IgG and IgM，the enzyme activities and mRNA expression levels of ALP，CK and LDH，and the mRNA expression of EGF-R in the intestinal mucosa.As shown in this study，the mRNA expression levels of digestive enzymes (ALP，CK and LDH) and EGF-R (P<0.05)，the activities of CK and LDH (P<0.01)，the levels of IgA，IgG and IgM (P<0.05)，the ADG (P<0.05) were significantly increased，and F/G(P<0.05) was significantly decreased in the recombinant pEGF-expressed S.cerevisiae group compared with the control and INVSc1(EV) groups.Herein，recombinant pEGF-expressed S.cerevisiae in this study have higher biological activities，including improving intestinal development and immune function，which will be applied in the swine industry.

中图分类号:

S828
S816.32

王蜀金，王斌星，郭春华，柏雪，彭忠利，张正帆，周琳. 分泌表达pEGF的重组酿酒酵母在断奶仔猪上的应用研究[J]. 畜牧兽医学报, 2016, 47(5): 944-954.

WANG Shu-jin，WANG Bin-xing，GUO Chun-hua，BAI Xue，PENG Zhong-li，ZHANG Zheng-fan，ZHOU Lin. Application Research of Recombinant EGF-expressed Saccharomyces cerevisiae in Early-weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 944-954.

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分泌表达pEGF的重组酿酒酵母在断奶仔猪上的应用研究

Application Research of Recombinant EGF-expressed Saccharomyces cerevisiae in Early-weaned Piglets

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